The Mount Isa AFL competition will be down to four clubs this year after Queensland AFL revoked Lake Nash Young Guns licence to compete in 2020.
The decision follows controversial incident in two games in 2019.
In August Tigers forfeited a game after they said their complaints to AFL Queensland about Lake Nash "verbal abuse" behaviour in a previous game went unanswered.
Then the semi-final between Tigers and Lake Nash was abandoned with ten minutes left on the clock and Tigers winning comfortably.
Multiple fights broke out on the field and spectator got involved before umpires called the game off, and Tigers were declared the victors with score at 108-26.
In a statement to the North West Star, AFL Queensland have confirmed they have taken action as a result of the two games.
"Following a game on the 17th of August 2019, Lake Nash AFC were found guilty of having partaken in socially unacceptable behaviours and were formally warned that any future incidents involving socially unacceptable behaviour from players, support staff or spectators of Lake Nash AFC would result in the immediate removal of the club from the AFL Mount Isa competition," the AFL Queensland statement read.
"The Club was deemed to have again partaken in socially unacceptable behaviours during their Preliminary Final match on the 7th of September 2019".
AFL Queensland said Lake Nash's licence suspension would be reviewed at the end of the 2020 season.
"Lake Nash players are still eligible to play with other licensed clubs in the Mount Isa competition," AFL Queensland said.
That leaves premiers Tigers facing off against Buffs, Rovers and Rhinos this season.
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