The Katter's Australian Party and One Nation will place each other as the number two position on their how to vote cards in the state election.
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KAP leader Robbie Katter said the Liberal and Labor major party dominance had failed regional Queenslanders.
"For far two long the major parties have continued to sell off our assets, pushed green ideology and failed to invest in regional job creating mega-infrastructure projects," Mr Katter said.
"Queensland can't afford another four years of major party dominance, we know it doesn't work, enough is enough, times up."
Pauline Hanson said there had always been a synergy between One Nation and Katter's, particularly when it comes to regional matters like strong support for coal mining and the farming sector.
"For the first time in political history, we're seeing Labor and the LNP preference the Greens, which most regional Queeslander's are horrified at the thought of," Senator Hanson said.
"Regional Queenslanders are crying out for proper representation at this election and if the Katter's can help One Nation secure seats and vice versa, that will be a significant win for the regions, particularly those in the western and northern parts of the state."
Mr Katter said the KAP would put the Greens last in all seats.
"The LNP are preferencing the Greens which highlights how out of touch they have become," he said.
"If there is a result of the LNP deal that ends up with a Greens controlled government this looks like no dams, no coal and more crime. We can't risk the Greens grabbing a seat, it's a major threat to the future of North Queensland."
The Greens have already grabbed a seat, winning the south-east seat of Maiwar in the 2017 election and are targetting other seats including that of former deputy premier Jackie Trad in South Brisbane
Mr Katter said other Westminster democracies where there was not two party dominance had better governance of their citizens.
"Stronger minor parties means politicians have to actually fight for your vote in order to make a difference," he said.
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