Queensland's border closure to Western Australia's coronavirus hotspots will be indefinite, with police checking all incoming flights for anyone trying to sneak in.
Travellers from the Perth, Peel and South West regions of WA must quarantine for 14 days upon arrival after a hotel quarantine worker tested positive for COVID-19.
Health Minister Yvette D'Ath hasn't put a time frame on the measures, saying they will remain flexible.
"We will wait for the information coming out of Western Australia to identify when we can lift those restrictions," she said.
Ms D'Ath said 47 people who arrived on a flight from Perth on Monday morning, and a small number of people who arrived from South Australia, had been put into quarantine.
Queensland police warned travellers that all incoming flights, not just those from WA, will be checked for people coming from coronavirus hotspots.
"So please if you're coming from Western Australia, if you're in a hotspot and you want to come here for a holiday, now's not the time to do that," Gold Coast Chief Superintendent Mark Wheeler said.
He said officers would also randomly pull over cars and trucks at road borders to check for anyone coming from WA hotspots.
Chief Supt Wheeler said people already in Queensland who had entered the state after 1am on January 25 needed to self-isolate and get tested.
Despite the state shutting to WA hotspots, Sydneysiders are again free to travel to the Sunshine State in a boost to the tourism industry.
The state's road border checkpoints with NSW have mostly been dismantled after police started taking them down at 1am on Monday.
Chief Supt Wheeler said officers had gained vast experience setting up and manning checkpoints twice over a period of 292 days, intercepting 1.2 million vehicles and turning away 14,600.
"We are now well practised in that, unfortunately, so if that was the case that we had to do it again we would do it within 24 hours," he said.
The removal of border checkpoints will ease domestic travel, but Acting Premier Steven Miles is concerned about the impact of international border closures on tourism.
He understands that Australia's borders need to remain shut, but repeated calls for JobKeeper to be extended past March for businesses catering to international tourists.
Federal Treasurer Josh Frydenberg seemed to rule that out on Monday, instead calling for tourism operators to adapt and be flexible.
Mr Miles said it was short-sighted to make Queensland's international tourism businesses change or go under.
"Those remarks from Minister Frydenberg - they're heartless, they're cruel," he told Seven's Sunrise program.
"I saw this morning he told businesses they should find a new way to make a profit, and that's just unrealistic for many of these tourism products which are designed for international tourists.
"And while many of them of course will change their businesses, will do other things, our tourism industry will need those products when international tourism returns."
Australian Associated Press